Blood stream infections are leading causes of mortality and morbidity throughout the world. The rapid identification of pathogens causing sepsis enable early initiation of appropriate antimicrobial therapy. Matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry has been established as a rapid reliable method for identification of cultured bacterial and fungal isolates. However, conventional processing requires overnight incubation of the blood culture broth on solid media prior to MALDITOF MS analysis, delaying organism identification, Methods: 530 positive blood culture bottles were analyzed MALDI-TOF MS by direct In-house methods and results were compared to results obtained by conventional culture on sold media Results: among 530 positive blood cultures, the in-house method correctly identified 74% of cultures to the species level and 16% to genus level and 10% gave no identification. Correct identification rate among Gram negative organisms was 96 % (196/204) and 88.8 % (286/322) among Gram positive organisms. Conclusion: This study demonstrated the effectiveness of Inhouse method for identifying bacteria directly from positive blood culture bottles. We propose our in-house method as an efficient and successful method to reduce time of identification of causative agent of bacteremia/sepsis