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Molecular diagnosis of three outbreaks during three successive years (2018, 2019, and 2020) of Lumpy skin disease virus in cattle in Sharkia Governorate, Egypt
Faculty
Veterinary Medicine
Year:
2022
Type of Publication:
ZU Hosted
Pages:
Authors:
Abdelkarim Mansour Moursy
Staff Zu Site
Abstract In Staff Site
Journal:
Open Veterinary Journal Faculty of Veterinary Medicine University of Tripoli , Libya
Volume:
Keywords :
Molecular diagnosis , three outbreaks during three
Abstract:
Background: Lumpy skin disease (LSD) is endemic in Egypt despite the Egyptian authorities’ annual mass vaccination of cattle with sheeppox vaccine (Veterinary Serum and Vaccine Research Institute, Egypt), and the LSD virus (LSDV) continues to thrive practically every summer. The disease has a huge economic impact on the trade of the animal and its by-product. Aim: This paper study the molecular characterization of LSDV strains that have been circulating in Sharkia Governorate, Egypt, for three successive years (2018, 2019, and 2020). Methods: A total of 61 specimens (26 skin nodules and 35 oculonasal swabs) were collected from a clinic in the hospital of veterinary medicine, Zagazig University, during the summer months (July, August, and September) of three outbreaks in 2018, 2019, and 2020. These were examined by polymerase chain reaction (PCR) based on the open reading frame 103 (ORF103) gene to confirm the suspected cases and determine the degree of homology between the three different outbreaks during three successive years between each other and between the derived sequences of GenBank. Results: Cattle is thought to be infected with LSDV due to the presence of scattered local or diffuse circumscribed skin nodules along with fever and lymph node enlargement and sometimes leg edema. The PCR approach proved rapid, sensitive, and specific for the detection of LSDV and confirmative diagnosis of the disease. Forty-six were detected to be positive by PCR (75.4%). The seven sequenced samples were translated to amino acid and registered in GenBank under accession number MW357655-MW357661. A single nucleotide mutation and amino acid variation were observed at positions 161 C (2020)/T (2018&2019) and consequently, a change in the amino acid at position 54 P (2020)/L (2018&2019) between the outbreak in 2020 and those in 2018 and 2019, respectively. The field LSDV isolates from Egypt cattle were more closely related to other LSDV sequences from Africa (Kenya), Asia, Europe, and the United States. These findings highlight the necessity of ongoing surveillance and characterization of circulating strains and the need to improve procedures for distinguishing vaccine strains from field viruses.
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Abdelkarim Mansour Moursy, "Prevalence and some risk factors with therapeutic trial of sheep dermatophytosis in Egypt", College of Veterinary Medicine / University of Mosul, 2022
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Abdelkarim Mansour Moursy, "Clinical and histopathological studies on neurodegeneration and dysautonomia in buffalo calves during foot-and-mouth disease outbreaks in Egypt", Veterinary World, 2021
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Abdelkarim Mansour Moursy, "Oxidative Stress Biomarkers in Dystrophic Lambs and Kids in Relation to Body Condition Scores المؤشرات الحيوية للإجهاد التأكسدي في الحملان وصغار الماعز المصابة بالاضمحلال العضلي وعلاقته بتصنيفات حالة الجسم", Zagazig University, 2017
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Abdelkarim Mansour Moursy, "Molecular and Phylogenetic Analysis of Canine Parvovirus Variants (2A-2B-2C) in Egypt التحليل الجزيئي والتطور الوراثي لمتغيرات فيروسات parvovirus (2A-2B-2C) في الكلاب في مصر", Nexuus academic publishers, 2019
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Abdelkarim Mansour Moursy, "Suppurative Injection Site lesion In Cattle: Clinico-biochemical, Ultrasonographic and histopathological examinations", Zagazig University, 2019
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Department Related Publications
Ahmed Mahmoud Wasfi Elkabany , "فصل مكونات بروتين السيرم في عجول الجاموس حديثة الولاده المصابه بالاسهال بواسطة الالكتروفوريزس ( التثريد الكهربي )", لايوجد, 1900
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