Abstract: |
In modernaquaculture,enrichingNiletilapia’sdietwithomega-3poly-unsaturatedfatty
acids (PUFAs)notonlyplaysanimportantroleinitsgeneralhealthbutalsofortifiesitsfilletwith
omega-3-PUFAs.However,themajorchallengeaffectingtheirdeliveryistheirhighinstabilitydueto
oxidative deterioration.Thus,theprospectiveincorporationofomega-3-PUFAsintonanocarriers
can enhancetheirstabilityandbioactivity.Inthisregard,theeffectofreformulatedomega-3-NPs
was investigatedonNiletilapia’sperformance,fleshantioxidantstability,immunity,anddisease
resistance.Fourfishgroupssupplementedwithomega-3-PUFAs-loadednanoparticles(omega-
3 NPs)atlevelsof0,1,2,and3g/kgdietandattheendoffeedingtrialfishchallengedwith
Aeromonashydrophila. Fishperformance(weightgainandfeedconversion)wasimprovedingroups
supplemented withomega-3-NPs(2and3g/kgdiet).Thedepositionofomega-3-PUFAsinfish
flesh elevatedwithincreasingdietaryomega-3-NPs.Simultaneouslytheoxidativemarkers(H2O2,
MDA, andreactiveoxygenspecies)infishfleshwerereduced,especiallywithhigheromega-3-NPs.
Post-challenge, downregulationofIL-1, IL-6,IL-8,TNF-, andcaspase-1werenoticedafterdietary
supplementation ofomega-3-NPs.Moreover,mRNAexpressionofautophagy-relatedgeneswas
upregulatedwhilethemTORgenewasdownregulatedwithhigheromega-3NPslevels.Lower
expressionof A. hydrophila ahyI andahyRgenesweredetectedwithomega-3NPssupplementation.
In conclusion,omega-3-NPsapplicationcanfortifytilapiafleshwithomega-3-PUFAsandaugment
its performance,immunity,anddiseaseresistanceagainst Aeromonashydrophila.
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