Nowadays, ultrasound has become an important diagnostic tool, and its applications are proving more robust and tremendously beneficial in different aspects of veterinary practices. The advent of ultrasonography enables researchers and veterinarians to assess and monitor tremendous physiological and pathological events in the farm, companion, and wild animals (1). Because there is a growing demand for use of ultrasonography in studying, diagnosis, and monitoring many physiological events and diseases of animals, the goal of this special edition Research Topic is to shed light on the progress made in the past decade in applications of diagnostic ultrasonography in different aspects of physiological and pathological issues in the veterinary practices (reproduction, internal medicine, surgery, and cardiology). In this e-collection, 14 articles were published covering the aforementioned objectives and to provide direction and guidance to researchers in the field.