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Assessment of Cephalopina titillator, different prepared antigens in diagnosis by indirect ELISA in serum and mucus of camels
Faculty
Veterinary Medicine
Year:
2021
Type of Publication:
ZU Hosted
Pages:
Authors:
Eman Ahmed Ali Hasanien
Staff Zu Site
Abstract In Staff Site
Journal:
EVMPSJ Egyptian Veterinary Medical Society of Parasitology
Volume:
Keywords :
Assessment , Cephalopina titillator, different prepared antigens , diagnosis
Abstract:
Cephalopina titillator infestation cause great economic losses where the infested camels show different clinical signs which varied from loss of appetite, decrease in body weights, and milk production to impaired breathing. In the current study, the prevalence of C. titillator was recorded by PM examination and indi r ect ELISA in serum and mucus samples as 26.27%, 83.33%, and 51.67%, respectively. Also, the study showed a highly significant relation between the sample sources (serum/mucus) and the antigen types (Excretory Secretory Products ESP, Organ 's content Co, Crude Cr & Somatic So Ags) depending upon the obtained res u l ts b y indirect ELISA. The usage of different antigens showed a significant variation in the obtained results by ELISA test, where Co Ag recorded the highest values followed by ESP and finally, So Ag showed the lowest values. The recorded results for indirect ELISA showed that the use of Co Ag for antibody detection in s erum samples showed the highest significant values in comparison with other used Ags . Area under Curve (AUC) referred to a good diagnostic accuracy of ELISA test where it reached 0.73 for Co Ag and ESP. The obtained results illustrated moderate to high significant agreement between PM examination and ELISA test. The stu d y concluded that using an indirect ELISA against different third instar antigens o f C. titillator reported higher values in serum than mucus. The strength of antigen immunogenicity was arranged as: Co > Cr > ESP > So in serum, and it was ESP > Cr > Co & So in mucus. Also, the use of Co Ag for the serum samples in ELISA considered the most immunogenic one which enhance IgG production and recommends its use for early and accurate serodiagnosis of C. titillator infestat io n in naturally infected camels.
Author Related Publications
Eman Ahmed Ali Hasanien, "Morphology and molecular characterization of Haemonchus contortus vulvar morphotypes from sheep at Sharkia Province, Egypt", الجمعيه الطبية البيطرية المصرية لعلم الطفيليات, 2013
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Eman Ahmed Ali Hasanien, "IDENTIFICATION OF FORENSICALLY IMPORTANT INSECTS ON ATRAZINE-INTOXI-CATED RAT CARCASSES AT DIFFERENT DECOMPOSITION STAGES DURING SUM-MER SEASON", computting. Si, 2021
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Eman Ahmed Ali Hasanien, "Diversity, Succession pattern and colonization of forensic entomofauna on indoor rat carrions concerning the manner of death", Accademia Nazionale dei Lincei 2021, 2021
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Eman Ahmed Ali Hasanien, "Seroprevalence and risk factors associated with Toxoplasma gondii infection in domestic rabbits and humans", Elsevier, 2017
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Eman Ahmed Ali Hasanien, "Light and scanning electron microscopy of Gasterophilus intestinalis (larvae and adult fly) infesting donkeys with emphasis on histopathology of the induced lesions.", Egyptian Veterinary Society of Parasitology, 2018
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Department Related Publications
Osama Mohamed Elshafei Elazazy, "التاثير المتبادل بين البلهارسيا والسسستيسركس فاشيولارز فى الجرزان البيضاء", لايوجد, 1900
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Osama Mohamed Elshafei Elazazy, "التاثير المتبادل بين البلهارسيا والسسستيسركس فاشيولارز فى الجرزان البيضاء", لايوجد, 1900
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Osama Mohamed Elshafei Elazazy, "التاثير المتبادل بين البلهارسيا والسسستيسركس فاشيولارز فى الجرزان البيضاء", لايوجد, 1900
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Osama Mohamed Elshafei Elazazy, "بعض الدراسات على الديدان الأسطوانية التى تصيب الحيوانات البرية آكلات اللحوم وعلى الاخص وبائيتها فى مصر ", لايوجد, 1900
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Enas Amien Mohamed Desouki , "الديدان المفلطحة فى الطيور البرية المصرية مع وصف 4 أنواع منها ", لايوجد, 1900
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