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atg7 and beclin1 are essential for energy metabolism and survival during the larval-to-juvenile transition stage of zebrafish
Faculty
Science
Year:
2022
Type of Publication:
ZU Hosted
Pages:
Pages 359-372
Authors:
Susan Attia Moawad
Staff Zu Site
Abstract In Staff Site
Journal:
Aquaculture and Fisheries ELSEVIER
Volume:
Volume 7,
Keywords :
atg7 , beclin1 , essential , energy metabolism , survival
Abstract:
The high mortality in larval-to-juvenile transition is a big problem in aquaculture which is related to the shifting from endogenous to exogenous feeding. However, the underlying causes remain poorly understood. Autophagy, an evolutionary regulated cellular mechanism, is highly conserved in eukaryotic organisms to maintain energy homeostasis against stress including starvation. To investigate whether autophagy plays a role during larval-tojuvenile transition, we generated atg7 and beclin1 zebrafish mutant lines using CRISPR/Cas9 gene editing technology. In this study, both atg7 and beclin1 null zebrafish died during larval-to-juvenile transition because atg7 and beclin1 mutants were unable to cope with the metabolic stress after yolk absorption and fail to activate autophagy in response to nutrient restriction. Meanwhile, dramatic defects in the intestine architecture and metabolic functions in the liver of these mutants were observed even though refeeding them. Treatment with rapamycin, an activator of autophagy, could effectively extend the survival time of both atg7 and beclin1 null zebrafish through decreasing the metabolic rate while it couldn’t activate autophagy in mutants via the canonical pathway. Thus, our results revealed that autophagy played a crucial role in zebrafish ontogeny during larval-tojuvenile transition, and it could be considered as one of the most important endogenous factors judging the survival rate of the developing embryos during this period.
Author Related Publications
Susan Attia Moawad, "A Comparative Morphological and Histological Study of the Olfactory Organ in Two Models of Bony Fishes (Mugil cephalus and Malapterurus electricus): A light and Electron microscopic study.", مجلة كلية العلوم, 2013
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Susan Attia Moawad, "strategy of Hepatic Metabolic Defects Induced by beclin1 Heterozygosity in Adult Zebrafish", china, 2020
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Susan Attia Moawad, "Dietary Supplementation of Nile Tilapia (Oreochromis niloticus) With Panax ginseng Essential Oil: Positive Impact on Animal Health and Productive Performance, and Mitigating Effects on Atrazine- Induced Toxicity", Frontiers, 2022
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Susan Attia Moawad, "Benefits of Chlorella vulgaris against Cadmium Chloride-Induced Hepatic and Renal Toxicities via Restoring the Cellular Redox Homeostasis and Modulating Nrf2 and NF-KB Pathways in Male Rats", MDPI, 2023
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Susan Attia Moawad, "In ovo protective effects of chicoric and rosmarinic acids against Thiacloprid-induced cytotoxicity, oxidative stress, and growth retardation on newly hatched chicks", Elsevier, 2023
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Department Related Publications
Samir Abdullah Taha, "Impact of plant extracts as molluscicides agent against Biomphalaria. alexandrina snails and Schistosoma mansoni", كلية العلوم, 2013
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Mahmoud Abdulaaty AbdelMoneim, "LIGHT AND SCANNING ELECTRON MICROSCOPIC STUDIES ON THE BODY SURFACE OF VARROA JACOBSONI OUDEMANS, 1904 (An ectoparasitic mite of the honeybee).", Egyptian Journal of Zoology, 1998
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Reham Zakaria Mustafa Mohammed Hamza, "Hepatoprotective and nephroprotective effects of Al-Taif Pomegranate (Punicagranatum L) extract against toxicity induced by Atrazine and Malathion pesticides in male albino mice", JOCPR, 2014
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Reham Zakaria Mustafa Mohammed Hamza, "Laboratory evaluation of garlic oil against the saw toothed beetle Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae), and its effect on serotonin and acetylcholinesterase levels", Global journal, 2015
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Abeer Samir Mohammed Yamani, "تأثير تغذية الطور اليرقى و البالغ على القدرة على البقاء و الاعاشة والخصوبة للبعوض ناقل حمى الضنك الايدس ألبوبكتس سكوز ( ثنائية الأجنحة: كيوليسيدى)", Journal of the Egyptian Society of Parasitology, 2015
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