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Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers
Faculty
Veterinary Medicine
Year:
2022
Type of Publication:
ZU Hosted
Pages:
Authors:
Heba Ahmed Abdallah Abdelmgeed
Staff Zu Site
Abstract In Staff Site
Journal:
Veterinary Sciences MDPI
Volume:
Keywords :
Genetic Relatedness, Antibiotic Resistance, , Effect , Silver Nanoparticle
Abstract:
In this study, we determined the prevalence and toxin types of antibiotic-resistant Clostridium perfringens in chicken, pigeons, camels, and humans. We investigated the inhibitory effects of AgNPs on biofilm formation ability of the isolates and the genetic relatedness of the isolates from various sources determined using RAPD-PCR. Fifty isolates were identified using PCR, and all the isolates were of type A. The cpe and cpb2 genes were detected in 12% and 56% of the isolates, respectively. The effect of AgNPs on biofilm production of six representative isolates indicated that at the highest concentration of AgNPs (100 µg/mL), the inhibition percentages were 80.8–82.8%. The RAPD-PCR patterns of the 50 C. perfringens isolates from various sources revealed 33 profiles and four clusters, and the discriminatory power of RAPD-PCR was high. Multidrug-resistant C. perfringens isolates are predominant in the study area. The inhibition of biofilm formation by C. perfringens isolates was dose-dependent, and RAPD-PCR is a promising method for studying the genetic relatedness between the isolates from various sources. This is the first report of AgNPs’ anti-biofilm activity against C. perfringens from chickens, pigeons, camels, and humans, to the best of our knowledge.
Author Related Publications
Heba Ahmed Abdallah Abdelmgeed, "Bayesian Estimation of Diagnostic Accuracy of Three Diagnostic Tests for Bovine Tuberculosis in Egyptian Dairy Cattle Using Latent Class Models", MDPI, 2021
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Heba Ahmed Abdallah Abdelmgeed, "Occurrence, genotyping, shiga toxin genes and associated risk factors of E. coli isolated from dairy farms, handlers and milk consumers", Elsevier Ltd., 2016
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Heba Ahmed Abdallah Abdelmgeed, "A comparative evaluation of PCR- based methods for species- specific determination of African animal trypanosomes in Ugandan cattle", BioMed Central Ltd., 2013
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Heba Ahmed Abdallah Abdelmgeed, "Seafood a potential source of some zoonotic bacteria in Zagazig, Egypt, with the molecular detection of Listeria monocytogenes virulence genes", Istituto G. Caporale 2013, 2013
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Heba Ahmed Abdallah Abdelmgeed, "Cleavage site stability of Egyptian highly pathogenic avian influenza viruses in backyard chickens during 2009-2011", Elsevier B.V, 2014
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Department Related Publications
Amal Anis Mahdi Eid, "Outbreaks of infectious laryngotracheitis in native hybrid", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, "التقييم المعملي للقاحات مرض التهاب غدة فابريش ضد فيروس المرض الضاري", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, "دراسات عن عترات الباستيريلا مالتوسيدا في مزارع البط جدارة التحصين الذاتي", لايوجد, 1900
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Ahmed Mohamed Alsadek Masoud Hegazyi, "The picture of immune response in chickens vaccinated with ND vaccines ", لايوجد, 1900
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Ahmed Mohamed Alsadek Masoud Hegazyi, "Viral agents associated with rertarded growth in broiler chickens. AM. Hegaz", لايوجد, 1900
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