This study was examined to investigate the effect of Azomite (AZO) on the growth performance, immune function, and bone mineralization of broiler chickens. A total of 240-d old male chicks were randomly assigned into four treatments with six replicates (n¼10), which included control (basal diet), basal diet þ0.25% AZO, basal diet þ 0.50% AZO and basal dietþkitasamycin as antibiotic growth promoter (AGP). The results indicate that live body weight (LBW), average daily gain (ADG) and feed conversion ratio (FCR) were significantly (p<.05) improved in AZO 0.25% and 0.50% than the control. The weight of bursa of Fabricus was significantly (p<.05) higher in AZO-0.25% and AZO-0.50% than control. Total protein (TP), globulin, IgA and IgG levels were significantly (p<.05) increased with AZO supplemented treatments. Tibia diameter tibia breaking strength was significantly (p<.05) increased in AZO- 0.25% and AZO-0.50% treatment. In conclusion, the results indicated that addition of AZO at the doses of 0.25% and 0.50% in the diet had beneficial effects on growth performance, immune functions and tibia breaking strength