This investigation aimed to improve the hatchery seed production strategy in terms of chronologies of embryonic and lar- val development as well as hatching, growth and survival rates, and rearing water quality of green tiger prawn, Penaeus semisulcatus by the integration effects of different water temperatures (23 °C, 26 °C, 29 °C, 32 °C) and probiotic as water additives. The best and shortest chronologies of embryonic development stages were in favor of 32 °C with probiotic. The best hatching rate was in favor of 29 °C with probiotics. The fastest larval development and greatest larval growth as total length until PL1 stage was promoted by 32 °C with probiotic but the best survival rate was recorded at 26 °C with probiotic followed by 29 °C with probiotic treatment. During larval development, the lowest value of NH4–N was recorded under the effect of 26 °C and probiotic. Therefore, probiotics as water additives at 29 and 32 °C water temperatures have a beneficial impact on the hatchery seed production strategy and quality of P. semisulcatus. Our findings could apply to rearing larvae in shrimp hatchery to decrease the chronology of embryonic and larval development, while increase the hatching rate, growth performance, and survival rates as well as improving the water quality.