Protoplasts are plant cells whose cell walls have been removed mechanically or enzymatically and, due to their totipotency and immediate response to external stimuli, have been considered an attractive plant explant for in vitro culture [1, 2]. In the last decades, there has been an increase in research using protoplasts, extending the genetic base, increasing productivity or resistance to pathogens of species of economic interest through production of somatic hybrids and, especially, through the advance of direct genetic transformation and genetic editing