In the present study the chemical composition and identification total phenolic content (TPC) and healthy and nutritional benefit of strawberry (Fragaria x ananassa) leaves powder was carried out to determine the important polyphenolic composition, potential of the strawberry leaves for potential biological applications. Diabetic rats were fed on strawberry leaves powder at percent(0.50%, 1% and 1.50% ). Blood glucose, lipid profile of rats, liver and kidney functions were determined in blood serum. Histopathalogical studies on liver, pancreas and kidney sections were carried out. The elevated levels of bloodglucose (221.11g/dl), TG (140.98), TC (144.88), LDL-C (70.765), (mg/dl) and the lowest level of HDL-C (39.915 mg/dl) were noted in group 2 animals(G2positive control ) at six weeks also three weeks compare with rats groups fed on strawberry leaves powder at the differences percentage . Results showed that strawberry leaves powder were found to lower the serum cholesterol, triglyceride, LDL-C, but were found to increase the HDL-C as compared to the corresponding diabetic group (positive control). The histopathological examination of liver, pancreas and kidney were more or less normalized in the groups that were fed strawberry leaves powder rats compared to the positive control group. Thus, the study demonstrates that strawberry leaves powder possesses a diabetic effect on serum lipid profile, liver, pancreas and kidney functions. Results are revealed an improve in liver, pancreas and kidney functions of rats fed on strawberry leaves powder.From the foregoing results in the current work, it could be recommended to using of strawberry leaves powder or extract at a ratio 0,5% 1.0% and 1.50% of differences fields such as manufacturing ornutrition specific diabetic to get the highest healthy and nutritional benefits of this leaves.