The research aims to identify the effect of eating royal jelly on: 1- Iron level among middle-distance runners. 2- The effect of iron level on some biochemical variables in the research sample. Statistical manipulations: * SMA. * Mediator. * standard deviation * skewness coefficient * correlation coefficient. *Mann Whitney test. * Wilcoxon test. * Percentage improvement. Among the most important results: 1- Eating royal jelly has a positive effect with statistically significant differences on the biochemical variables (iron - hemoglobin - red blood cells - hematocrit - transferrin- ferritin) among the members of the research sample. 2 - There are percentages of improvement between the tribal and remote measurements in favor of the post measurement in all the biochemical variables of the long-distance runners, where the hematocrit variable in the blood recorded the lowest percentage of improvement, which amounted to (2.43%), and the ferritin variable recorded the highest improvement rate, which amounted to (13.81%). The most important recommendations were: 1- The need to pay attention to eating royal jelly in improving iron levels in the blood of middle-distance runners. 2- Paying attention to the iron levels of medium -distance runners, while recording and following-up the biochemical variables for each runner because of their positive effects on the runners’ athletic performance. 4- Taking the nutritional balance into consideration during the preparation of the training programs because it is of great importance in achieving the results and the digital levels of the contestants.