Background: Helicobacter pylori (H. pylori) is a common organism in developing countries, it causes gastric disorders and cancer. Pathogenesis of these disorders involve cytokine gene polymorphisms that affect cytokine levels and clinical diseases. Objective: The aim of the study is to identify the relationship of IL-1β-511, IL-10-519 and TNF-α-308 polymorphisms to the risk of H. pylori infection and occurrence of gastric disorders. Methods: IL-1β-511, IL-10- 519 and TNF-α-308 polymorphisms were assessed using polymerase chain reaction (PCR) restriction fragment length polymorphism technique (RFLP) in 356 subjects classified according to H. pylori infection and gastric disorders. Results: Carriers of T allele of IL-1β-511 and IL-10-519 had increased risk of H. pylori infection (OR:1.95, 95% CI:1.4-2.7, P< 0.001). Simultaneous occurrence of either IL-1β-511 TT or IL-10-519 TT genotypes with H. pylori significantly augmented the risk for different gastric diseases (gastritis; P=0.005 & 0.002, PU; P=0.01&0.02 and GC; P=0.02&0.01; respectively). While, the copresence of TNF-α-308 GA+ AA genotypes and H. pylori was related to gastritis only. Conclusions: This study revealed a significant association of the IL-1β - 511C/T and IL-10-819C/T but not TNF-α308 G/A polymorphisms with risk of Helicobacter pylori infection and different gastric diseases in Egyptian patients.