Background: Polycystic ovarian syndrome is an endocrine disorder that can affect adolescent females. Screening and awareness are important initial steps in PCOS management. Health education of the population at risk is crucial to health-seeking behavior that can improve quality of life. Aim: Screening for features of PCOS and determining the effect of health-education on PCOS awareness among female university students. Method: This study was of a pre-post research design. The current study involved 900 childbearing age females, who were randomly selected from students of Zagazig University. Data were collected using a structured questionnaire. PCOS screening was based on anthropometric measurements, as well as Rotterdam criteria. A structured lecture on PCOS was received by all participants. Results: The participants’ mean age was 19.01±0.7 years. Three-quarters of them had no information about PCOS. Less than one quarter of them was sufficiently aware of PCOS pre educational while 84.0% of them were sufficiently aware after the educational program. Conclusion: The participants’ awareness was improved after PCOS structured educational program. Recommendation: Early screening and inclusion of PCOS in the student’s curriculum are therefore recommended.