Background: Asthma is a chronic respiratory illness marked by inflammation and reversible obstruction of the airways, which may cause Wheezing, shortness of breath, dyspnea and cough. Aim and objectives: Detect relationship between IL-8 -781C/T (rs2227306) polymorphism and pediatric asthma. Subjects and Methods: This study was a case control study included 40 asthmatic pediatric patients and 40 healthy controls of matched age and sex in the period from June 2020 to December 2020 at Outpatient clinics, Pediatric Department at Zagazig University Hospitals. Results: There is a statistically significant difference between the cases and control groups examined when it comes to level of IL-8 and Interleukin 8 -781C\T Polymorphism. CC and CT genotypes increased risk of asthma by 8.67 and 3.76 folds respectively. C allele increased that risk by 3.38 folds. Conclusion: Our findings support IL8's inflammatory function and suggest that it may be utilized as a criterion for the severity of asthma in children. More studies on the IL8 gene variants and their biological effects are also required. Keywords: inflammatory, lung function, genotypes, Asthma, GINA, IL8 gene.