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HPLC and Chemometric Methods for the Simultaneous Determination of Miconazole Nitrate and Nystatin
Faculty
Pharmacy
Year:
2012
Type of Publication:
Article
Pages:
855-861
Authors:
Mostafa, Samia, Salama, Ismail, El-Sadek, Mohamed, Heneedak, Hata M
DOI:
10.1093/chromsci/bms127
Journal:
JOURNAL OF CHROMATOGRAPHIC SCIENCE OXFORD UNIV PRESS INC
Volume:
50
Research Area:
Biochemistry \& Molecular Biology; Chemistry
ISSN
ISI:000310819400002
Keywords :
HPLC , Chemometric Methods , , Simultaneous Determination , Miconazole
Abstract:
High-performance liquid chromatography (HPLC) and chemometric methods were applied to the simultaneous determination of the two nonsteroidal antifungal drugs, miconazole (MIC) and nystatin (NYS). The applied chemometric techniques are multivariate methods including classical least squares, principal component regression and partial least squares methods. The ultraviolet (UV) absorption spectra of the standard solutions of the training and validation sets in methanol are recorded in the range of 280-320 nm at 0.2-nm intervals. The HPLC method depends on reversed-phase separation using a C18 column. The mobile phase consists of a mixture of methanol-acetonitrile-ammonium acetate buffer (pH 6; 50 mM) (60:30:10 v/v/v). The UV detector was set at 230 nm. The developed methods were validated and successfully applied to the simultaneous determination of MIC and NYS in their tablets. The assay results obtained using the chemometric methods were statistically compared to those of the HPLC method and good agreement was observed.
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