Wheat is one of the most important cereals, vital basic crop in Egypt, covering approximately 32.6% of the total winter land. However, sensitivity to abiotic stress especially salinity is one the major current hurdles that reduce the global yield of this crop. Plant biopriming with fungi is the recent technology that might alleviate the salt stress effect. Thus, the objective of this work was to examine the effect of Triticum aestivum grains biopriming with Phanerochaete chrysosporium on the response of wheat to salt stress. From the physiological and biochemical parameters, biopriming of wheat grains with P. chrysosporium significantly alleviates the salt stress and markedly increases the growth parameters, photosynthetic pigments and osmolytes (soluble sugars, soluble protein and proline) contents. In addition, it alleviates the oxidative damage, as indicated by the lower accumulation of malondealdehyde and increasing the activity of antioxidant enzymes; superoxide dismutase, catalase, and ascorbate peroxidase in wheat seedlings. Results indicate the potential of using P. chrysosporium biopriming for reducing the deteriorating effects of salinity