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Molecular Characterization of Thymidine Kinase and Glycoprotein G Genes from a Possible Vaccine Induced Infectious Laryngotracheitis Outbreak in Egypt
Faculty
Veterinary Medicine
Year:
2014
Type of Publication:
ZU Hosted
Pages:
Authors:
Abeer Mohamed Mohamed Shaahin
Staff Zu Site
Abstract In Staff Site
Journal:
Pak Vet J, Pak Vet J,
Volume:
Keywords :
Molecular Characterization , Thymidine Kinase , Glycoprotein , Genes
Abstract:
Infectious laryngotracheitis virus (ILTV) is a worldwide cause of acute respiratory disease in chickens. In this study, an outbreak of laryngotracheitis in commercial layers flock, Sharkia Province, Egypt in 2011 was investigated by sequencing of thymidine kinase (TK) and glycoprotein G (gG) genes. The chickens showed clinical ILTV infection, histopathological examination revealed epithelial sloughing, development of syncytical cells, heterophilic exudation with presence of eosinophilic intranuclear inclusion bodies. The virus was isolated on chorioallantoic membrane (CAM) of embryonated chicken eggs (ECE), and it induced typical pock lesions after two passages. The obtained 647 amplicon by PCR confirmed the presence of ILTV genome. Gene sequencing of TK and gG showed high genetic homology between field isolate from commercial layer flock (Sharkia-11) and vaccine strains. To our knowledge, this is the first documented ILTV outbreak attributed to vaccination with live modified ILT vaccine in Sharkia, Egypt based on sequence analysis of TK and gG genes.
Author Related Publications
Abeer Mohamed Mohamed Shaahin , "Some Studies on Swollen Head Syndrome in Broiler Chickens in Egypt", Zagazig Veterinary Journal, 2017
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Abeer Mohamed Mohamed Shaahin , "First Report of Duck Hepatitis A Virus 3 from Duckling Flocks of Egypt", aaap, 2020
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Department Related Publications
Amal Anis Mahdi Eid, "): Infectious bronchitis virus infection in Egypt International Symposium on infectious bronchitis and pneumovirus ", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, "دراسات عن مرض الارانب النزيفي في مصر التغيرات الباثولوجيه للعدوي الطبيعيه والتجريبيه", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, ":دراسه مبدئيه لاستخام زيت حبه البركه في انتاج الدواجن", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, ":تغيرات ميكرو فلورا الامعاء في الدجاج البلدي أثناء الرقاد الطبيعي", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, ":دراسه عن مرض الارانب النزيف في محافظه الشرقيه", لايوجد, 1900
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