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Isolation and Molecular Identification of Duck Hepatitis A Virus in Sharkia Governorate
Faculty
Veterinary Medicine
Year:
2018
Type of Publication:
ZU Hosted
Pages:
Authors:
Abeer Mohamed Mohamed Shaahin
Staff Zu Site
Abstract In Staff Site
Journal:
Zagazig Veterinary Journal Zagazig Veterinary Journal
Volume:
Keywords :
Isolation , Molecular Identification , Duck Hepatitis , Virus
Abstract:
Thirty two flocks were examined to investigate the incrimination of DHAV in the nervous signs and massive mortalities recorded among ducklings during 2014-2016 in Sharkia Governorate. The examined birds aged (2-11 days) were suffering from nervous signs; ataxia followed by opisthotonos, kicking spasmodically then rapid death. Enlarged liver with marked petechial haemorrhages, enlarged spleen, nephritis and swelling of the kidneys were seen. Viral isolation in ECE revealed stunting, subcutaneous hemorrhages covering the body, necrotic areas and greenish discoloration of embryonic liver in 22 samples (68.75%). DHAV identification was succeeded by RT-PCR using RNA extracted from the livers of collected samples and specific primers based on 3D gene in all above positive samples. It could be concluded that RT-PCR could be carried out on hepatic tissues for rapid diagnosis of common DHAV. The subtyping of DHAV is useful for accurate diagnosis to avoid vaccination failure.
Author Related Publications
Abeer Mohamed Mohamed Shaahin , "Some Studies on Swollen Head Syndrome in Broiler Chickens in Egypt", Zagazig Veterinary Journal, 2017
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Abeer Mohamed Mohamed Shaahin , "The Role of Infectious Bronchitis Virus in Respiratory and Renal Problems in Broiler Chickens", Zagazig Veterinary Journal, ©Faculty of Veterinary Medicine, 2018
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Abeer Mohamed Mohamed Shaahin , "Molecular Characterization of Thymidine Kinase and Glycoprotein G Genes from a Possible Vaccine Induced Infectious Laryngotracheitis Outbreak in Egypt", Pak Vet J,, 2014
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Abeer Mohamed Mohamed Shaahin , "First Report of Duck Hepatitis A Virus 3 from Duckling Flocks of Egypt", AAAP, 2020
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Abeer Mohamed Mohamed Shaahin , "First Report of Duck Hepatitis A Virus 3 from Duckling Flocks of Egypt", aaap, 2020
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Department Related Publications
Amal Anis Mahdi Eid, "Outbreaks of infectious laryngotracheitis in native hybrid", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, "التقييم المعملي للقاحات مرض التهاب غدة فابريش ضد فيروس المرض الضاري", لايوجد, 1900
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Ibrahiem Abdelrahman Ibrahiem Ghanem, "دراسات عن عترات الباستيريلا مالتوسيدا في مزارع البط جدارة التحصين الذاتي", لايوجد, 1900
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Ahmed Mohamed Alsadek Masoud Hegazyi, "The picture of immune response in chickens vaccinated with ND vaccines ", لايوجد, 1900
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Ahmed Mohamed Alsadek Masoud Hegazyi, "Viral agents associated with rertarded growth in broiler chickens. AM. Hegaz", لايوجد, 1900
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