This study aimed to investigate the effects of Nano-selenium (Nano-Se), organic selenium (Org-Se), vitamin E and their combination on semen quality and some blood parameters of New Zealand White bucks rabbits (NZW). A total number of 24 NZW with 2671± 185 initial body weight and 24 weeks of age were used in the study. The bucks were randomly allotted to three experimental main groups. The 1st group fed basal diet and served as a control. The 2nd group fed basal diet and orally treated with 0.5 mg Org- Se / kg body weight (BW). The 3rd group fed basal diet and orally treated with 0.5 mg Nano-Se /kg BW. The experimental groups were divided according to Vitamin E administration into two sub groups. The 1st sub group was left as a control (without vitamin E administration) and the 2nd sub group orally treated with 250 mg Vit. E/ kg Bw. Physical semen characteristics and hematological and biochemical blood parameters were studied. Mean values of physical semen characteristics were significantly higher (P < 0. 01) for buck rabbits fed diets with selenium sources and vitamin E as compared with those fed on basal diet (control). RBCs, WBCs, blood platelets, hematocrit, MCV and MCHC, were within the normal physiological range and the differences were not significantly affected by treatment with selenium sources. However, Hb, MCH and lymphocytes percentages were significantly increased (P < 0.05 and P < 0.01) for buck rabbit's fed basal diet with Nano selenium. The mean values of RBCs, Hb, hematocrit, MCHC and lymphocytes percentages were significantly higher (P < 0.05 and < P 0.01) in buck rabbits fed basal diet with vitamin E than un-treated rabbits. However, the mean values of blood platelets and neutrophils percentages were significantly higher (P <0.05 or P<0.01) in buck rabbits fed basal diet without vitamin E than those fed diet with vitamin E. Total protein and globulin were significantly increased (P<0.05 and P < 0.01) while, albumin and