In the last years, the concept of proper sustainability, cost potency of plant growth-promoting bacteria, and organic manures drive us to focus on their exploration in agriculture. Laboratory effectiveness of Serratia spp. and Pseudomonas spp. isolates against egg hatching and infective juvenile mortality of the root-knot nematode, Meloidogyne incognita was determined. As well as performance evaluation of composted chicken manure (CM) inoculated with bacteria under greenhouse and field conditions on vegetable plants, cucumber, pepper, potato and tomato besides banana. In vitro results after the 5ᵗʰ day of inoculation, S. marcescens (A10) and Pseudomonas fluorescens PF131 isolates gained the highest inhibition of egg hatching (85.18 and 75.36%). While juvenile mortality percentages were 43.30 and 35.10% with S. marcescens (A10) and P. putida (PP29), respectively. The nematicidal effect of the tested rhizobacteria on M. incognita inhibited egg hatching and juvenile mortality was directly proportional to isolates and exposure time. Under greenhouse conditions, the interaction between rhizobacteria and CM increased the fresh potato plant's shoot weight (31.78%) and root weight (28.94 %). Furthermore, the decrease in the number of galls, egg masses, IJs/100 g soil, and RF population significantly to 69.57%, 63.37%, 12.40, and 0.147, respectively. Under field conditions, the combination of chicken manure seems to be responsible for the sustainability of rhizobacteria in situ and extends their potency for a long period. After 20 days of the field application (1.8×10⁸ cfu/ml; 15 L /Fed), the reduction percentages in IJs in blocks treated with the mixture of rhizobacteria and CM were 88.94, 87.92, 91.27, and 9276 % in tomato, cucumber, pepper, and banana plants, respectively. Our results indicate that the chicken manure inoculated with rhizobacteria is a promising biocontrol agent mixture for control of M. incognita in infected vegetables and orchard fields heavily infested.