ABSTRAC T Background: Non-alcoholic fatty liver disease (NAFLD) is one of the most common diseases in the world, as it is associated with bad food habits. While no specific treatment for NAFLD, coffee takes renewed interest on progression of disease. Aim of the work: to study the possible prophylactic effect of Arabic coffee and its combination with atorvastatin on experimentally induced non-alcoholic fatty liver disease in adult male albino rats.Material and methods: 56 adult male albino rats were used and divided into 7 groups (G) each group 8 rats in number as the following:G1:control on normal diet , G2: NAFLD group induced by 2% cholesterol, and groups (G 3 –G7) NAFLD treated groups, G3: received atorvastatin ( 10 mg/kg/ once daily), G4: received coffee extract (100 mg/kg/twice daily), G5: received coffee extract (200 mg/kg/twice daily), G6: received coffee extract (400 mg/kg/twice daily), G7:received coffee extract (200 mg/kg/twice daily) + atorvastatin (10 mg/kg/once daily). NAFLD treated rats received drugs orally by gavage daily for 9 weeks starting at 5th week from onset of induction of NAFLD, after that blood samples were collected for biochemical analysis , and liver carefully excised and cut into two specimens; one for assessment of oxidative stress, the other liver specimen prepared for histopathological study. Results: Cholesterol 2% induced typical picture of fatty changes compared with control, evidenced by steatosis, ballooning and fibrosis in liver tissue stained by hematoxylin and eosin (H and E), and Mallory trichrome stains. The serum level of triglycerides (TG) along with liver index and aspartate aminotransferase (AST) significantly increased, and the serum level of high density lipoprotein (HDL) significantly decreased, hepatic level of glutathione peroxidase (GPx), GSH/GSSG ratio significantly reduced, while hepatic malondialdehyde (MDA), and serum level of pro-inflammatory cytokine a tumor necrosis factor- α (TNF-α) elevated significantly. In 200, 400mg/kg coffee extract treated rats, and coffee + atorvastatin treated rats, the hepatic fat and collagen markedly reduced, the serum level of triglyceride (TG), liver index, alkaline phosphatase and hepatic MDA also reduced significantly. The hepatic GSH/GSSG ratio, GPx enzyme activity in liver in the last three mentioned groups were significantly higher compared to NAFLD induced group, the TNF-α also decreased, and anti-inflammatory IL-10 increased in the previously mentioned groups. Conclusion: Arabic coffee extract has preventive effect on NAFLD and this effect due to its hypolipidemic, antioxidant, and anti-inflammatory activity, and its combination with atorvastatin exhibited synergistic effect on biochemical and histopathological features of NAFLD.