Two field experiments were carried out at a Private Farm in Qeft District, Qena Governorate, Egypt during the two consecutive seasons of 2016/2017 and 2017/2018. The present work aimed to study the effect of humic acid rates (0.0, 1.0, 2.0 and 3.0 l/fad.), sprays number (one, two, three and four times after (25, 40, 55 and 70 days, respectively, from transplanting date) and their combination treatments on vegetative growth, flower yield components and volatile oil production of chamomile (Matricaria chamomilla) plant. The obtained results referred to that the maximum values of plant height, branch number/plant, herb dry weight/plant as well as air-dry weight of flower heads/plant and /fad., were detected when chamomile plants were applied with the highest rate of humic acid and fertilized three times during the season, in most cases. In the same time, the treatment of humic acid rate at 3 l/faddan combined with three times addition was superior in volatile oil percentage in flower heads and volatile oil yield (ml/ plant and l/faddan) of chamomile compared to the other ones under study in the two cuts during both seasons. Generally, this combination treatment seems promising in the development of sustainable crop growth and yield advantages under Qena Governorate conditions.