Gamma irradiation has been proved to be an economical and effective mechanism for improving plant tolerance to abiotic stresses. Thus, two pot experiments were conducted at the green house of National Research Centre, Dokki, Cairo, Egypt, during two successive seasons to investigate the physiological role of γ- rays with three doses (15, 25 and 35Gy) for improving growth and some biochemical aspects of faba bean plants grown under salinity stress (6.24ds/m). The results showed that, marked decreases in growth criteria, photosynthetic pigments, total carbohydrates and total soluble protein contents in faba bean plants irrigate with saline water as compared with control plants. Meanwhile, increases in phenolics, hydrogen peroxide (H2O2), malonaldhyde (MDA) and some antioxidant enzyme activities (superoxide dismutase SOD, catalase CAT, peroxidase POX and ascorbic acid peroxidase ASP) infaba bean leaves. On the other hand, pre-sowing treatments of faba bean seeds by different γ- ray doses could alleviate the harmful effects of salinity stress via increasing markedly growth characters concomitantly with increases in the levels of photosynthetic pigments, total carbohydrates, total soluble protein and phenolic contents. Meanwhile, different treatments decreased hydrogen peroxide (H2O2), malonaldhyde (MDA). Antioxidant enzymes activities show variable responses, SOD and ASP activities increased meanwhile, CAT and POX activities decreased. Regarding the protein banding patterns based on SDS-PAGE, there were noticeable differences between treatments as a response to irradiation treatments and interaction of this irradiation with salinity, so, it appeared 18 bands (total number of bands) with molecular weights ranged from 41.162KDa to 18.659KDadistributed as: monomorphic bands (1 band), polymorphic bands (5 bands) and unique bands (12 bands).