Background: Universities include complex environments with a wide range of hazards, especially in research, that represent high risk on staff. However, little is known about university staff and employees' awareness of health hazards and their adherence to safety precautions in developing countries. This study aims to assess the levels of awareness of Zagazig University staff and employees towards occupational hazards and safety practice and study the relationship with their demographic and occupational data. Methods: A cross-sectional study was carried on 282 staff and employees (121 teaching staff 112 employees and 49 hospital health care workers) at Zagazig University, using a structured questionnaire to collect data. Results: Results indicated that the majority (86.7%) of the studied group were aware of psychosocial hazards, followed by mechanical, chemical, biological, and physical (69.1%, 64.2%, 51.8%, and 42.2%) respectively. Only 55.7% of the studied group were aware of control measures. Conclusion: High percentage of university staff and employees lack awareness about occupational hazards. They also lack awareness about occupational safety practice and control measures. Keywords: university staff, occupational hazards, safety practice