Adjuvant effect of saponin in an oil-based monovalent (serotype O) foot-and-mouth disease virus vaccine on the antibody response in guinea pigs and cattle

Faculty Science Year: 2021
Type of Publication: ZU Hosted Pages:
Authors:
Gamal EL Daydamony Mohamed Bioumy
Journal: Archives of Virology volume Springer Nature Volume:
Keywords : Adjuvant effect , saponin , , oil-based monovalent (serotype    
Abstract:
In this study, the combined effect of Montanide ISA 50 and saponin was evaluated. Two experiments were performed in guinea pigs and one in cattle to determine the optimal antigen and saponin doses. Only serotype O of foot-and-mouth disease virus (O/PanAsia-2 of ME-SA topotype) was employed in preparation of the monovalent vaccine. All animals were immunized twice with a four-week interval, except for the negative controls. Blood was collected 10 days after the second booster, and the immune response was evaluated using a serum neutralization test.
    
     
 
       

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