Modulating the gut microbiota has been proposed as a potential strategy for improving host health and productivity and avoiding the undesirable effects on gut health and the immune system. In ovo technology, through which a tiny quantity of material is injected into the bird’s egg/embryo during incubation, offers a novel alternative for delivering bioactive compounds to an embryo before hatching. Recent experiments from various researchers showed the benefits of in ovo feeding technology on the chicken body weight, feed conversion ratio, and pectoral meat yield. Using early-life programming via the in ovo technique with different feed additives may be possible to avoid selected the metabolic disorders, poor immunity, and pathogen resistance.