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Environmental science and pollution research
Springer
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Abstract: |
There is cumulative evidence that iprodione (IPR) fungicide and chlorpyrifos (CPF) insecticide are endocrine disruptors that can
evoke reproductive toxicity. Yet, the underlyingmechanisms are still unclear. Besides, the outcomes of their co-exposure to male
sexual behavior and male fertility are still unknown. The effects of IPR (200 mg/kg b.wt) and CPF (7.45 mg/kg b.wt) single or
mutual exposure for 65 days on sexual behavior, sex hormones, testicular enzymes, testis, and accessory sex gland
histomorphometric measurements, apoptosis, and oxidative stress biomarkers were investigated. In addition, expression of
nuclear receptor subfamily group A (NR5A1), 17β-hydroxysteroid dehydrogenase (HSD17B3), silent information regulator
type-1 (SIRT1), telomerase reverse transcriptase (TERT), and peroxisome proliferator-activated receptor gamma coactivator 1-
alpha (PGC-1α) genes has been assessed. Our results revealed that the individual or concurrent IPR and CPF exposure significantly
disturb the sexual behavior, semen characteristics, testicular enzymes, and male hormones level. Oxidative stress caused
by IPR and CPF activates apoptosis by inducing Caspase-3 and reducing Bcl-2. Downregulation of HSD17B3, NR5A1, and
SIRT1/TERT/PGC-1α pathway was evident. Of note,most of these disturbances were exaggerated in rats co-exposed to IPR and
CPF compared to IPR or CPF alone. Conclusively, our findings verified that IPR and CPF possibly damage the male reproductive
system, and concurrent exposure should be avoided.
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