ABSTRACT Aim: To study the intraurethral dexpanthenol (DXP) effect on urethral wound healing after urethroplasty in a rat model. Methods: 54 rats were distributed into 3 groups; control, urethroplasty, and DXP groups. Urethroplasty was occured by creating a 0.8-cm vertical midline urethral incision with a microknife, then closing the urethra and overlying corpus spongiosum using 7-0 PDS. 0.9% saline solution and DXP (500 mg/kg) was then applied intraurethrally once a day till sacrification to urethroplasty and DXP groups, respectively. Six rats were sacrificed on the postoperative days 4, 8 and 15 from each group. Penectomy was performed and the penises were then sent for histopathological examination, and nitric oxide (NO) and malondialdehyde (MDA) activity assessment. Results: There was significant improvement in inflammation and fibrosis scores after intraurethral DXP application for 8 and 15 days. DXP group showed significant reduction in MDA and NO activity on days 4, 8 and 15. Apart from histopathology, and intraurethral application of DXP up to 15 days significantly provided more beneficial effects. Conclusion: Administration of intraurethral DXP post urethroplasty reduces urethral wound healing.