In this study, we evaluated the antibacterial and antibiofilm activity of AgNPs against P.aeruginosa which isolated from dental implant. Previous studies revealed the presence of opportunistic pathogens such as P. aeruginosa, S. aureus and yeast, and showed the high affinity of these pathogens for titanium surfaces . According to previous studies, patients have a greater pocket depth, bone loss, more bacterial load, and a high prevalence of the disease. In this study, we isolated 3 p. aeruginosa isolates out of 30 samples recovered from dental- implant infections from patient with different ages at the clinics of faculty of Dentistry, Zagazig University, Zagazig, Egypt. P.aeruginosa characterized by its ability to grow in cetrimide agar, and produce oxidase enzyme. Identification was confirmed using VITEK 2 system. Previous studies indicated that, after implant placement, the periodontal pathogens are early transmitted from periodontal to implant sites . In our study; resistance of isolated bacteria to 16 antibiotics was estimated. All isolates were resistant to 13 (81%) out of 16 antibiotics. In this study, the isolated bacteria showed exhibited a higher capacity for biofilm formation. Current study showed that the AgNPs can increasingly inhibit the growth of p.aeruginosa involved in dental plaque biofilms. In conclusion, the needy for an additional tool for the clinical management of bacterial infections, is urgent. Nanoparticles offer a promising alternative therapy to antibiotics, and the research on it has been promoted since the emergence of AMR.