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Extensive Drug-Resistant Salmonella enterica Isolated From Poultry and Humans: Prevalence and Molecular Determinants Behind the Co-resistance to Ciprofloxacin and Tigecycline
Faculty
Veterinary Medicine
Year:
2021
Type of Publication:
ZU Hosted
Pages:
Authors:
Norhan Khayry Abdelaziz Mahmoud
Staff Zu Site
Abstract In Staff Site
Journal:
Frontiers in Microbiology Frontiers
Volume:
Keywords :
Extensive Drug-Resistant Salmonella enterica Isolated From Poultry
Abstract:
The emergence of extensive drug-resistant (XDR) Salmonella in livestock animals especially in poultry represents a serious public health and therapeutic challenge. Despite the wealth of information available on Salmonella resistance to various antimicrobials, there have been limited data on the genetic determinants of XDR Salmonella exhibiting co-resistance to ciprofloxacin (CIP) and tigecycline (TIG). This study aimed to determine the prevalence and serotype diversity of XDR Salmonella in poultry flocks and contact workers and to elucidate the genetic determinants involved in the co-resistance to CIP and TIG. Herein, 115 Salmonella enterica isolates of 35 serotypes were identified from sampled poultry (100/1,210, 8.26%) and humans (15/375, 4.00%), with the most frequent serotype being Salmonella Typhimurium (26.96%). Twenty-nine (25.22%) Salmonella enterica isolates exhibited XDR patterns; 25 out of them (86.21%) showed CIP/TIG co-resistance. Exposure of CIP- and TIG resistant isolates to the carbonyl cyanide 3-chlorophenylhydrazone (CCCP) efflux pump inhibitor resulted in an obvious reduction in their minimum inhibitory concentrations (MICs) values and restored the susceptibility to CIP and TIG in 17.24% (5/29) and 92% (23/25) of the isolates, respectively. Molecular analysis revealed that 89.66% of the isolates contained two to six plasmid-mediated quinolone resistance genes with the predominance of qepA gene (89.66%). Mutations in the gyrA gene were detected at codon S83 (34.62%) or D87 (30.77%) or both (34.62%) in 89.66% of XDR Salmonella. The tet(A) and tet(X4) genes were detected in 100 and 3.45% of the XDR isolates, respectively. Twelve TIG-resistant XDR Salmonella had point mutations at codons 120, 121, and 181 in the tet(A) interdomain loop region. All CIP and TIG co-resistant XDR Salmonella overexpressed ramA gene; 17 (68%) out of them harbored 4-bp deletion in the ramR binding region (T-288/A-285). However, four CIP/TIG co-resistant isolates overexpressed the oqxB gene. In conclusion, the emergence of XDR S. enterica in poultry and humans exhibiting CIP/TIG co-resistance with no previous exposure to TIG warrants an urgent need to reduce the unnecessary antimicrobial use in poultry farms in Egypt.
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Norhan Khayry Abdelaziz Mahmoud, "Class 1 integron and associated gene cassettes mediating multiple-drug resistance in some food borne pathogens", Malaysia, 2016
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Department Related Publications
Mohammed Naser Mohamed Ali, "Survival of Foot and Mouth disease virus strain O, / 2 /72 in Egypt outside the animal body", لايوجد, 1900
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Heba Ahmed Abdallah Abdelmgeed, "A comparative evaluation of PCR- based methods for species- specific determination of African animal trypanosomes in Ugandan cattle", BioMed Central Ltd., 2013
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Maysa Abdelbadea Ibrahiem Awaadallah , "تأثير بعض المطهرات الكيميائية شائعة الاستخدام و الأشعة فوق البنفسجية على خمسة من الملوثات الفطرية ذات الاهمية البيطرية والصحية واسعة الانتشار فى مساكن الماشية", Fac. of Veterinary Med. Zagazig University, Egypt, 2010
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