Concentrations of heavy metals in agricultural land near highways are a major concern for humans. This study was conducted to investigate the contamination level of heavy metals in soil, canola crop, and the potential health risk for honeybee and human. The average concentrations (mg/kg) of Co (15.94), Cr (169.66), Ni (55.39), Mn (765.34) Hg (2.99), and Cu (51.31) were elevated beyond their background reference values in world soil average, while Pb (9.45) was below to their respective background levels. This was confirmed by contamination factor (CF) and ecological risk factors (Er). Heavy metal concentrations in different parts of canola decreased in the following order: Fe> Mn > Cr > Pb > Co > Cu > Ni > Hg. Honey transfer factor (TFH) of heavy metals was less than unity except Ni and Hg. Human health (non-carcinogenic) risk assessment of heavy metals in the soil through potential exposure pathway (ingestion) recorded a dramatically increased risk for children (hazard index, HI=2.44). Hazard quotient via honey (HQH) consumption value of heavy metals were within the safe limits (HQ< 1). Probably, honeybees have a strong ability to transfer Co, Pb, Hg, and Mn (HQ> 1) from the canola to their hives during collecting pollen and nectar. HQ in honeybee workers from the consumption of honey can be used to derive HQ in humans using the hazard factor (HF). HF is 1481.482 (Pb), 2356.902 (Ni), and 3888.889 (Cr), respectively, for adult human (70kg) and 317.460 (Pb), 504.377(Ni), and 832.22 (Cr) for children (15kg).