Petroselinum sativum (PS) belongs to Apiaceae family and is widely used as herb, spice and vegetable. We aimed to investigate the anti-urolithiatic effect of PS extract against ethylene glycol inducing urolithiasis and kidney stones in rats. Serum levels of creatinine, urea, uric acid, sodium, potassium, calcium and magnesium before and after treatment were measured. Protein gel electrophoresis was also assessed. Serum uric acid and creatinine were significantly decreased, magnesium level was significantly increased and calcium level was significantly decreased in treated groups compared with the group induced by ethylene glycol only without receiving treatment. Histopathology showed significant decrease in calcium oxalate deposits in kidneys of the treated groups compared with the ethylene glycol group. Protein gel electrophoresis showed significant decrease in both low and high molecular weight proteins in treated groups and significant increase in high molecular weight proteins in the group induced by ethylene glycol