Impact of animal saliva on the performance of rapid antigen tests for detection of SARS-CoV-2 (wildtype and variants B.1.1.7 and B.1.351)

Faculty Veterinary Medicine Year: 2021
Type of Publication: ZU Hosted Pages:
Authors:
Ibrahim Mohamed Sabet Sabet
Journal: Veterinary Microbiology Elsevier Volume:
Keywords : Impact , animal saliva , , performance , rapid antigen    
Abstract:
SARS-CoV-2 infects several animal species and SARS-CoV-2 variants of concern (VOCs) may even show (as in humans) enhanced inter- and intra-species transmission rates. We correlated sensitivity data of SARS-CoV-2 rapid antigen tests (RATs) to viral RNA genome equivalents analyzed by real-time reverse transcriptase- polymerase chain reaction (RT-PCR). Further, we checked their suitability for testing animals by assessing saliva and VOC effects. Viral loads up to 2 logs (RNA copy number) under the hypothetical SARS-CoV-2 infec-tivity threshold were detected by most analyzed RATs. However, while saliva from various animal species showed generally no adverse effects on the RATs’ analytical sensitivities, the detection of VOCs B.1.1.7 and B.1.351 was in some RATs inferior to non-VOC viruses
    
     
 
       

Author Related Publications

  • Ibrahim Mohamed Sabet Sabet, "Detection of SARS-CoV-2 variant B.1.1.7 in a cat in Germany", Elsevier, 2021 More
  • Ibrahim Mohamed Sabet Sabet, "Pathogenicity of Highly Pathogenic Avian Influenza Virus in naturally infected poultry in Egypt", PLOS ONE, 2015 More
  • Ibrahim Mohamed Sabet Sabet, "Abrogation of Marek’s disease virus replication using CRISPR/Cas9", Springer Nature, 2020 More
  • Ibrahim Mohamed Sabet Sabet, "Cas9-expressing chickens and pigs as resources for genome editing in livestock", National Academy of Sciences, 2021 More
  • Ibrahim Mohamed Sabet Sabet, "Marek’s disease virus: from novel viral interleukin-8 (vIL-8) splice variants to inhibition with CRISPR/Cas9", Freie Universität Berlin, 2020 More

Department Related Publications

  • Ibrahim Mohamed Sabet Sabet, "Detection of SARS-CoV-2 variant B.1.1.7 in a cat in Germany", Elsevier, 2021 More
  • Gamelat Kottb Farag Kotb, "H5N1 Avian Flu Infection in Hubbard Broiler Chicken Can Be Prevented or Cured by Methylated Soy Protein During 42 Days Rearing", Springer, 2021 More
  • Shimaa Mohamed Galal, "Avian paramyxovirus type 1 in Egypt: Epidemiolog, Evolutionary, Perspective, and Vaccine Approach", Frontiers, 2021 More
  • Fakry Fahmy Mohamed Fakry, "Avian paramyxovirus type 1 in Egypt: Epidemiolog, Evolutionary, Perspective, and Vaccine Approach", Frontiers, 2021 More
  • Shimaa Mohamed Galal, "Molecular Characterization of Thymidine Kinase and Glycoprotein G Genes from a Possible Vaccine Induced Infectious Laryngotracheitis Outbreak in Egypt", Pakistan, 2014 More
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