Background: High rate of methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage among health care workers (HCWs) represents a major risk factor for hospital acquired infections (HAIs). Objectives: Our objectives were to determine the rate of MRSA nasal carriage among HCWs in Surgery Department in our hospital, to investigate the antibiotic susceptibility pattern for MRSA isolates and to assess the effectiveness of mupirocin for eradication of MRSA. Methodology: A cross sectional study was conducted on 150 HCWs. Nasal swabs were collected for detection of MRSA isolates and their antimicrobial susceptibility pattern by standard bacteriological procedures. Results: The carriage rate of MRSA was 14.6%. Nurses showed a significantly higher carriage rate. Using mupirocin, 70% of MRSA carriers were decolonized. Conclusion: High rate of nasal carriage of MRSA among HCWs in our surgery department necessitate application of proper infection control measures.