The present study aimed to assess the biosorption capacity of live, autoclaved and dried biomass of Yarrowia lipolytica AUMC 9256 for cadmium (Cd(II)). Minimum inhibitory concentration 500 mg/L proved great tolerance of Y. lipolytica AUMC 9256 to Cd(II). The time course growth in the presence of different concentrations of Cd(II) was studied. For live, autoclaved and dried biomass, maximum uptake capacities accomplished at pH 5.0, initial metal ion concentration 400 mg/L, biomass dosage 1g/L, and contact time 20min for autoclaved and dried biomass and 240 min for live cells. The potential of Y. lipolytica to produce cadmium nanoparticles was determined by UV-Visible spectroscopy measurements and transmission electron microscopy examinations. Moreover, to identify the possible mechanisms of Cd(II) uptake, TEM examinations, Fourier Transform Infrared spectroscopy, Energy dispersive X-ray microanalysis, and X-ray powder diffraction analyses were carried out. The potential implementation of dried Y. lipolytica biosorbent for heavy metal removal from different water samples was successfully accomplished using the multistage microcolumn technique. Accordingly, Y. lipolytica AUMC 9256 can be considered as a very promising potential to bioremediate Cd(II) ions and biosynthesis of Cd NPs.