Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Utility of polymerase chain reaction for rapid diagnosis of paediatric fungal keratitis: A comparative study with conventional mycological work up
Faculty
Medicine
Year:
2021
Type of Publication:
ZU Hosted
Pages:
10
Authors:
Ghada Abdelmoneiem Mokhtar Ahmed
Staff Zu Site
Abstract In Staff Site
Journal:
Microbes and Infectious Diseases Microbes and Infectious Diseases
Volume:
Keywords :
Utility , polymerase chain reaction , rapid diagnosis
Abstract:
Background: Fungal keratitis (FK) is a serious infection with bad complications in paediatric population, thus, rapid diagnosis is necessary. Early diagnosis is a beneficial tool necessary for better management and prognosis of FK and also for prevention of subsequent complications such as endophthalmitis, amblyopia ,and loss of vision . In recent years, polymerase chain reaction (PCR) became the predominant diagnostic method for mycotic keratitis, being frequently employed for complementing microbiological approaches. The aim of this study to determine the risk factors of paediatric fungal keratitis and evaluate the utility of PCR in diagnosis by comparing its sensitivity and specificity with conventional fungal culture (the gold standard). Methods: Corneal scrapings obtained from clinical suspected patients with FK and subjected to direct microscopic examination with 10% KOH, Gram smear, fungal culture and conventional PCR using common probe to all fungi. Results: The most prominent predisposing factor for FK was ocular trauma (n=40, 57%) followed by vernal keratoconjunctivitis (n=14, 20%). Out of the 70 studied cases featuring presumed fungal keratitis, 35 were culture positive (50%), with Fusarium spp. (48.5%) and Aspergillus spp. (20%) were the predominant isolates. By comparing fungal culture (the gold standard) versus 10 % KOH examination, Gram smear, and PCR were 57.1%, 28.6%, and 97.1%, respectively. Conclusion: Ocular trauma and vernal keratoconjunctivitis are the most common risk factors for pediatrics FK with Fusarium spp. and Aspergillus spp. are the most common isolated fungi. Polymerase chain reaction is a sensitive technique for diagnosing paediatric patients with FK
Author Related Publications
Ghada Abdelmoneiem Mokhtar Ahmed, "CMV, B and C hepatitis among multitransfused hereditary hemolytic Anemia children: an updated Egyptian experience", italian journal of pediatric, 2021
More
Ghada Abdelmoneiem Mokhtar Ahmed, "Genetic polymorphism of vitamin D receptors and plasminogen activator inhibitor-1 and osteonecrosis risk in childhood acute lymphoblastic leukemia", molecular genetic and genomic medicine, 2021
More
Ghada Abdelmoneiem Mokhtar Ahmed, "Vitamin D deficiency and vitamin D receptor gene polymorphisms as a risk factor for severe early-onset neonatal sepsis", Alexandria Journal of Pediatrics 2018,, 2018
More
Ghada Abdelmoneiem Mokhtar Ahmed, "Comparison between Specific Immunoglobulin E and Skin Prick Test for Diagnosis of Allergic Patients", Egyptian Journal of Medical Microbiology, 2020
More
Ghada Abdelmoneiem Mokhtar Ahmed, "Fungal profile of otomycosis in a sample of Egyptian patients in Zagazig university hospitals: A prospective study", Microbes and Infectious Diseases, 2021
More
Department Related Publications
Ashwak Mohamed Foad Abotaleb, "دراسة الاصابة بالميكروبات الحلزونية البوابية في مرضي السكر بالمقارنه بغير المصابين به", لايوجد, 1900
More
Hanan Mohamed Elsayed Ibrahiem, "-Prevalence ofHCV,HBV and HIV among blood donors in Sharkia", لايوجد, 1900
More
Refaat Abdelsamiea Sadek, "Association between antibody to hepatitis C virus and HBs Ag in blood donors", لايوجد, 1900
More
Refaat Abdelsamiea Sadek, "Wheezing-associated respiratory viral infections in early life. Acceptance for Publication", لايوجد, 1900
More
Abdallah Abdelkader Albeely, "الفيروس الغدى و التهاب الجهاز التنفسى فى الاطفال", لايوجد, 1900
More
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف