Two field experiments were carried out at Experimental Farm of Fac. Of Environ. Agric. Sci.,Arish Univ., El-Arish, North Sinai Governorate, Egypt during the two successive winter seasons of 2017/2018 and 2018/2019, to study the effect of foliar spray of proline and humic acid with some organic fertilizers on growth, productivity and oil content of Matricaria chamomilla L. The plants were fertilized with chicken manure and farmyard manure at (0, 10 or 20 m3 fed.-1 of each). All the above-mentioned fertilization treatments received calcium super phosphate (15.5%P2O5) at 200 kg fed.-1 and potassium sulphate (48% K2O) at 50 kg fed.-1. While foliar spray with humic acid and proline at (0, 50,100 or 150 mgl-1). The obtained results showed that chicken manure addition at 20 m3 fed.-1with humic acid foliar spray at 150 mgl-1 recoded the highest values for all growth parameters as well as yield components (expressed as fresh weight of yearly flower heads/ plant, dry weight of yearly flower heads/ plant and yield of yearly flower heads/ feddan). Also, the same treatment recoded the highest values of the major components (which expressed as farnesene, α- Bisobolol Oxide B, α-Bisobolol, chamazuline and α-Bisobolol Oxide A) of chamomile oil (%) compared to the other treatments under study.