Background: The possible health effects of radio frequency modulated electromagnetic fields (RF-EMF) emitted by mobile phones, cordless phones, base stations and Wi-Fi routers are a growing concern for the public around the world. Aim of the study: to study the possible effects of electromagnetic radiation from mobile phones on oxidant and antioxidant status of testicular tissue and testicular function of male albino rats and its underlying mechanism. Materials and Methods: Twenty male albino rates were divided into two groups: control group A (vehicle driving) and rats were exposed to the phone when the phone was turned off. Exposure to mobile radiation in group B (call mode). Group B was exposed to electromagnetic frequencies for 60 minutes every day for 6 weeks. Results: Mobile radiation exposure in male rats resulted in a significant decrease in serum levels of FSH, LH, testosterone, epididymal sperm …