OBJECTIVE: The aim of this study was to investigate the possible effects of electromagnetic radiation from cell phone use on the oxidant and antioxidant status in testicular tissue and determine the possible protective role of vitamins D in modulating effect of chronic exposure to cell phone radiation on the testicular functions of male albino rats and its underlying mechanism MATERIALS AND METHODS: control group A (vehicle treated) andthe rats were exposed to the mobile phone while it was switched off. In Group B mobile radiation exposed (call mode) + vehicle treated and Group C: mobile radiation exposed (call mode) + oral administration of vitamin D by gavage (500 IU/Kg/day). Group B and C were exposed to the same electromagnetic frequency for 60 min daily for 6 weeks. RESULTS: Mobile radiation exposure in male rats induced significant decrease in serum FSH, LH, testosterone levels, epididymal sperm count, sperm motility with insignificant changes in either body weight gain or testicular weight, significant increase in testicular malondialdehyde (MDA) with significant decrease in testicular antioxidant SOD and GPx activity in comparison to control group and showed structural changes in the form of rupture of the basement membrane, dropped off spermatogenic cells, inter tubular fibrosis and leydig cells hyperplasia. However, administration of vitamin D resulted in a significant recovery of all the abovementioned parameters in the mobile radiation exposed rats' group. CONCLUSION:we confirmed the harmful effect of mobile phone radiations on testicular function in male albino rats, in addition, we confirmed the protective effect of Vitamin