Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia

Faculty Veterinary Medicine Year: 2018
Type of Publication: ZU Hosted Pages:
Authors:
Dina Mahmoud Ahmed Metwally
Journal: Plos ONE PUBLIC LIBRARY SCIENCE Volume:
Keywords : Gene-based molecular analysis , COX1 in Echinococcus granulosus    
Abstract:
The diversity and importance of Echinococcus species in domesticated animals in Saudi Arabia are poorly understood. In this study, 108 singular (hydatid) cysts were collected from goats (n = 25), sheep (n = 56) and camels (n = 27). DNA was extracted from the protoscoleces of individual fertile cysts and used for polymerase chain reaction (PCR) amplification of mitochondrial subunit 1 of the cytochrome c oxidase 1 (cox1) gene. Amplicon sequencing results revealed the presence of Echinococcus granulosus sensustricto (s.s.) (genotypes G1–G3) in 16 of the17 sheep cysts and 2 of the 27 camel cysts.of these samples, 18 (2 camel and 16 sheep) were divided into genotypes G1, G2, and G3.
    
     
 
       

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