Abstract Background: A crucial factor in delivering high-quality patient care is nursing implementation of evidence-based practice (EBP). Aim of this study: to explore nurses' attitudes, perceived self-efficacy, barriers, supporting factors, training needs, and information sources used to undertake different evidence-based practice activities in Zagzaig University Hospitals. Subjects and Methods: Research design: A descriptive correlational study design was utilized. Setting: this research was conducted at Zagazig University Hospitals. Subjects: A Stratified random sample of 384 nurses working at Zagazig University Hospitals. Tools of data collection: A questionnaire of perceptions of nurse‟s evidence-based practice (EBP) was used for data collection was developed by Majid et al (2011), it includes seven parts: 1) personal characteristics of the studied nurses. 2) Attitudes toward EBP, 3) level of self-efficacy, 4) supporting factors in adopting EBP, 5) barriers for adopting EBP, 6) training needs, 7) and sources of information used by the nurses .Results: the majority of nurses had unfavorable attitudes toward EBP, They preferred using traditional methods over changing to new approaches, and they perceived to possess moderate levels of skills to undertake different EBP activities. In addition, all nurses are facing many difficulties in implementing EBP. Conclusion: The majority of nurses had unfavorable attitudes toward EBP, They preferred using traditional methods over changing to new approaches, and perceived to possess moderate levels of skills to undertake different EBP activities. In addition, all nurses are facing many difficulties in implementing EBP. Recommendations: Encourage nurses to attend nursing conferences, scientific meetings, and involve them in the developmental activities, empower nurses to take active role in organizational change and implementation of evidence practice research findings, a reward system may be needed to enhance evidence based practice development and achievements in hospitals