The current study was conducted to assess fatty acids profile of Mugil cephalus fillets as affected by lowdose of commercial Gamma ray’s irradiation (0, 0.75, 1.5, 2.25 and 3 kGy). The results showed that the palmitic (c16:0) and stearic acids (c18:0) were predominant saturated fatty acids (SFAs) while, oleic (c18:1) and linoleic acids (c18:2) were the predominant unsaturated fatty acids (USFAs) in all treated and control samples. The concentration of total saturated (SFAs) and monounsaturated fatty acids (MUFAs) were incremented with increment irradiation dose. Oleic acid (c18:1) and Palmitoleic acid (c16:1) were major (MUFAs) in all treated and control samples of Mugil cephalus. Linoleic (c18:2), eicosapentaenoic (c20:5) and docosahexaenoic (c22:6) acids were predominant polyunsaturated fatty acid (PUFAs) regarding treated in addition to control samples. The Significant difference (p < 0.0 5) was found between irradiated and non-irradiated Mugil cephalus of some PUFAs (Linoleic, Arachidonic and Eicosapentaenoic acid).