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ROLE OF INSULIN GROWTH FACTORS (IGFS) IN MAMMALIAN BLASTOCYST DEVELOPMENT
Faculty
Medicine
Year:
2015
Type of Publication:
ZU Hosted
Pages:
Authors:
Adel Mohamed AbdelKhalek AbouRejaili,
Staff Zu Site
Abstract In Staff Site
Journal:
AIN SHAMS MEDICAL JOURNAL AIN SHAMS MEDICAL JOURNAL
Volume:
Keywords :
ROLE , INSULIN GROWTH FACTORS (IGFS) , MAMMALIAN BLASTOCYST
Abstract:
Background: Insulin growth factors (IGFs) are proved to be produced by the embryos and the maternal reproductive tract. Aim of the work: This study was conducted to spot a light on the role of IGFs on the preimplantation development of In Vitro cultured mice embryos. Material and Methods: This work was carried out on adult Cluster Differentiation 1 (CD1) strain pregnant mice that were sacrificed at the embryonic age of 2.25 day (E2.25) (at which the mice embryos are of 8- cell stage). Then, the embryos were collected from the oviducts and two sets of experiments were performed; IGF-I experiment: In which 18 embryos were transferred and cultured in a drop of KSOM medium (IGFI control group) and 18 embryos in another drop of KSOM medium enriched with 100 ng/ml IGF-I (IGF-I treated group). IGF-II experiment: In which 18 embryos were transferred and cultured in a drop of KSOM medium (IGF-II control group) and 18 embryos in another drop of KSOM medium enriched with 100 ng/ml IGF-II (IGF-II treated group). The four groups of embryos were left in a double gas incubator until E4.5 (the blastocyst-stage) at which all types of cells are well differentiated and express specific genes. Trophoectoderm (TE) cells express CDX2 gene, Epiblast (EPi) cells express NANOG gene, Primitive Endoderm (PrE) cells express GATA6 and SOX17 genes, apoptotic cells express Caspase 3 gene and the proliferative cells express Phosphohistone3 gene. These cells of the blastocyst were identified by immunohisto-chemistry technique using primary and secondary antibodies together with DAPI fluorescent stain for all cell nuclei. Thereafter, image analysis was done and each type of cells of the blastocyst was counted and statistically analyzed. Results: IGF-I and IGF-II produced significant decrease in the number of apoptotic cells together with a significant increase in the cell number of Inner Cell Mass (ICM), EPi cells and the total number of cells of the blastocysts compared with the corresponding values of the control groups. However, IGF-I and IGF-II displayed insignificant change in the number of proliferating cells and PrE cells of the blastocysts. Regarding the number of TE cells, IGF-I produced a significant increase, while IGFII produced insignificant change. Conclusion: IGFs have anti-apoptotic effect that was associated with an increase in the cell number of ICM particularly Epi cells together with an increase in the total number of cells of the blastocysts. These findings suggest an important role of IGFs as survival factors during the preimplantation period of the In Vitro cultured mice embryos.
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