Important gaps in knowledge remain regarding the potential of nanoparticles (NPs) for ‎plants, particularly the existence of helpful microorganisms, for instance, arbuscular mycorrhizal ‎(AM) fungi present in the soil. Hence, more profound studies are required to distinguish the ‎impact of NPs on plant growth inoculated with AM fungi and their role in NP uptake to develop ‎smart nanotechnology implementations in crop improvement. Zinc ferrite (ZnFe2O4) NPs are ‎prepared via the citrate technique and defined by X-ray diffraction (XRD) as well as ‎transmission electron microscopy for several physical properties. The analysis of the XRD pattern ‎confirmed the creation of a nanocrystalline structure with a crystallite size equal to 25.4 nm. The ‎effects of ZnFe2O4 NP on AM fungi, growth and pigment content as well as nutrient uptake of ‎pea (Pisum sativum) plants were assessed. ZnFe2O4 NP application caused a slight decrease in ‎root colonization. However, its application showed an augmentation of 74.36% and 91.89% in ‎AM pea plant shoots and roots’ fresh weights, respectively, compared to the control. Moreover, ‎the synthesized ZnFe2O4 NP uptake by plant roots and their contents were enhanced by AM ‎fungi. These findings suggest the safe use of ZnFe2O4 NPs in nano-agricultural applications for ‎plant development with AM fungi.‎