The Green Revolution faced a great cost to meet ever-increasing demands for food, where ‎indiscriminate use of agrochemicals resulted in non-friendly habitats. Therefore, the development ‎of a sustainable approach to better crop production of onion seeds (Allium cepa L.) is very ‎crucial. It is time to use organic waste as a replacement for agrochemicals by using arbuscular ‎mycorrhizal fungi (AMF) and Trichoderma. Fish waste as representative of food waste acts as a ‎leading cause of contamination of the environment. The interaction of AMF and Trichoderma ‎viride on biomass, total soluble protein, mycorrhizal colonization, amino acids, phosphatases and ‎phosphorus and nitrogen contents of onion plants grown in fish waste amended soil was studied. ‎Fish waste has caused a slight increase in onions biomass, total free amino acids, and soluble ‎protein content while with AMF and T. viride dual inoculation more increments were recorded; ‎such increases were related to an increase in mycorrhizal colonization. T. viride application ‎significantly increased the mycorrhizal colonization levels, but these were significantly reduced ‎with waste addition. Analysis of amino acids in plants showed that their concentrations had ‎changed as a result of waste addition combined with AMF and/or T. viride. The effectiveness of ‎fish waste combined with low cost and health/environmental safety leads to a prediction that the ‎introduction of fish waste coupled with fungi will become a more popular feature of agriculture ‎in the future.‎