This study aims to develop the kinematic effectiveness of 100 meter run in general and especially reduce maximum acceleration phase through the kinematic analysis of the 100 meter run segments and the phase of reduced maximum acceleration of the segment (70 – 80 meter), and the effect of the proposed training program on the kinematic variables. The researchers used the experimental method based on biomechanical analysis by using (5) cameras of frequencies 30 cadres/second and Simi Motion program for movement analysis on a sample of (3) international players of the Egyptian National Team, and every player perform (2) trials, meaning that there was (6) trials as a whole. The results revealed the most important kinematic variables of 100 meter run segments in general and the phase of Negative acceleration phase of the segment (70-80 meter) the Negative acceleration phase was developed for the longest distance which was improved from (72 meter) to (74 meter), the researchers recommend the guidance of the kinematic markers of the study and to use the specific training (Helper – resistance) to improve the length and frequency of the stride.