To study the interaction effects of humic acid rates x irrigation water quality on productivity of fodder beet yield (Beta vulgaris L.) and its quality as well as some soil chemical properties, two field experiments were conducted in a split plot design with three replications in two different location, El-Salam canal and Bahr Hadous drain in Sahl El-Hossinia ,El-Sharkia – Governorate , Egypt during to winter seasons (2015 and 2016). The studied factors were four rates of humic acid (0, 800 , 1600 and 2400 ml HA for 400 L-1 of irrigation water i.e, 0,2, 4 and 6 ml HA L-1, respectively) and two types of irrigation water (El-Salam canal water 1.75 dSm-1 and Bahr Hadous drain water 3.31 dSm-1). The results indicated that using each of irrigation water and humic acid rate gave the best values of all the studied characters in El-Salam canal region compared to that obtained from Bahr Hadous drain region. Concerning the interaction effect (El-Salam canal irrigation water x HA 6 ml L-1 rate) recorded the maximum values for all studied characters: growth attributes (root length , root diameter, FW , DW , FY , DY, TFY, TDY and total chlorophyll) , yield quality (CP, DCP, CF, ash, fat % in different organs and proline in top organs of fodder beet plants) and chemical composition ( N, P, K, Fe, Mn and Zn concentrations and uptake ) of fodder beet yield as well as some soil chemical properties ( pH, EC, CEC , macronutrients and micronutrients contents)