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Modulation of IR as a therapeutic target to prevent NASH using NRF from Diceratella elliptica (DC.) jonsell. Strong Nrf2 and leptin inducer as well as NF-kB inhibitor
Faculty
Pharmacy
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Amal Amien Mahmoud Elgendy
Staff Zu Site
Abstract In Staff Site
Journal:
phytomedicine Elsevier
Volume:
Keywords :
Modulation , , , , therapeutic target , prevent NASH using
Abstract:
Background: Insulin resistance (IR) and lipotoxicity were evidenced as the major nonalcoholic steatohepatitis (NASH) initiators. However, absence of the effective treatment against NASH progression raised our aim to discover a new promising insulin modulator and NSH preventer. Purpose: Our study aimed to extract and prepare a nitriles rich fraction (NRF) from Diceratella elliptica (DC.) Jonsell, investigate its insulin-sensitizing & anti-NASH potentialities and address its molecular targets in IR- NASH pathogenesis. Study design: NRF was prepared using natural autolysis method and compounds were identified. Then, seventy male Wistar rats were feed high fat diet (HFD) or normal pellets for 35 days. In day 14th, HFD rats were injected by Streptozotocin (STZ) once and treatment was started in day 21st with either NRF (30, 60 and 120 mg/kg; orally) or pioglitazone (PioG) (10 mg/kg; i.p) beside HFD. While, NRF-alone rats were treated with NRF (120 mg/kg; orally) beside the normal pellets. Body weight, glucose homeostasis, hepatopathological examinations were performed. Methods: Gas liquid chromatography-mass spectrophotometry (GLC/MS) was used for compounds’ identification while spectrophotometer was used for total glucosinolates (GLS) quantification. Also, the biochemical and molecular investigations concerned with liver lipotoxicity, oxidative stress, inflammation and insulin signaling pathway were investigated and confirmed with the computational prediction of the major compounds’ targets. Results: Butenyl and benzyl GLS were the major along with other volatile compounds. NRF had significantly increased the insulin sensitivity and improved NASH-hisptopathology showing hepatoprotective effect. While, the fraction’s anti-NASH potentiality was evidenced in the normalized hepatic steatosis markers, inflammation and oxidative stress key transcriptional factors resulting in induction of insulin receptor substrates (IRSs) phosphorylation and its downstream effectors. Conclusion: NRF has reversed IR, stimulated leptin secretion and prevented NASH initiation showing promising anti-NASH and anti-fibrotic effects
Author Related Publications
Amal Amien Mahmoud Elgendy, "A Pharmacognostical Study of Ochradenus Baccatus Del.Family ", لايوجد, 1900
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Amal Amien Mahmoud Elgendy, "Phytoconstituents, LC-ESI-MS Profile, Antioxidant and Antimicrobial Activities of Citrus x limon L. Burm. f. Cultivar Variegated Pink Lemon", Journal of Pharmaceutical Sciences and Research, 2017
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Amal Amien Mahmoud Elgendy, "Volatile Constituents, Antimicrobial and Cytotoxic Activities of Citrus reticulata Blanco Cultivar Murcott", International Journal of Pharmacognosy and Phytochemical Research, 2017
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Amal Amien Mahmoud Elgendy, "Phytoconstituents, LC-ESI-MS profile, antioxidant and antimicrobial activities of Citrus x limon L. Burm. f. cultivar variegated pink lemon", Journal of Pharmaceutical Sciences and Research (2017), 9(4): 375-391., 2017
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Amal Amien Mahmoud Elgendy, "Elicitation induced flavonoids, phenolic constituents, antioxidant and cytotoxic activities of Artemisia monosperma callus cultures", Journal of Medicinal Plants Research, 2016, 10, 717–731., 2016
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Department Related Publications
Ehsan Mahmoud Abdelaziz Abozied, "Pharmacognostical study and phytochemical investigation of launaea mucronata ", لايوجد, 1900
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Ehsan Mahmoud Abdelaziz Abozied, "7) GC-MS and Antimicrobial Activity of Volatile Oil of Apium Leptophyllum (Benth). ", لايوجد, 1900
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Ehsan Mahmoud Abdelaziz Abozied, "17) Phytochemical study and Bioassay of some constituents from Heliotropium Bacciferum Forssk", لايوجد, 1900
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Ehsan Mahmoud Abdelaziz Abozied, "27) Phytochemical and Biological studies of the shells of Arachis hypogae L. Fruits (Pea Nut) Cutivated in Egypt", لايوجد, 1900
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