In the existing study, substitution of different divalent metal ions in Li0.25Mn0.5-xMxFe2.25O4 (where, M = Co2+, Ni2+, Cu2+; x = 0.0, 0.1, 0.3 and 0.5) was performed to improve the magnetic properties. Their structural and magnetic features were examined by X-ray diffraction (XRD), scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM). XRD analysis proved the presence of single phase cubic spinel structure in all prepared compositions. The lattice parameter decreased linearly with increasing doping concentrations. The crystallite size values vary from 60 to 101 nm.